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He soon was deployed into the Ganado territory to work for Saddler in order to gain the Plaga samples from him, he was responsible for kidnapping Ashley Graham and encountered Leon twice, he was defeated by Leon and then finished off by Ada. Jack Krauser: Krauser is Leon's former partner and faked his death, then worked as Albert Wesker's most loyal henchman.

Within the Castle's Summit Tower, Leon defeated Salazar, his Verdugo bodyguards and the Queen Plaga. Salazar managed to jack Leon's Radio, and was successful in carrying out Ashley's ritual and transferring her to the Island Base.

Ramon Salazar: Salazar is the eigth castellan of the Salazar Family's Castle and ruled the Zealots with the Dominant Species Plaga.

Saddler moves Ashley to the Island Base, the location where Leon eventually defeated Saddler. He was responsible for injecting Leon and Ashley with Las Plagas, and soon revealed his plans to Leon within the Church.

Osmund Saddler: Saddler is leader of the Los Illuminados cult and controlled the Ganado with the Dominant Species Las Plagas.

But he ended up betraying Saddler, leaving notes on information of these monsters, as well as taking the samples and vaccines to help Leon and Ashley.

Luis Sera: Luis was an old Spanish researcher for Osmund Saddler, being responsible for the many creations of Plaga creatures such as Regenerators, Novistadors and the El Gigante.President in a Spanish Village, she was first imprisoned within the Los Illuminados Church. President's daughter and was kidnapped by Jack Krauser under the orders of Osmund Saddler. Ada Wong: After surviving the Raccoon City Incident, as a Double Agent, Ada now works for Albert Wesker to follow his orders and gain the sample, but her true goals is to give the Dominant Species Plaga to her true employees.President, Leon's first assignment was to rescue Ashley Graham from Osmund Saddler's cult and has been force to eliminate the attacking Villagers. Government Agent, under the direct orders of the U.S. Leon Scott Kennedy: Now a specially-trained U.S.Leon then meets another Human survivor, Luis Sera, who was captured by the Villagers and later Leon discovers each Villager are "Ganado", violent people under the control of whoever wields the Dominant Species Plaga Parasite and he finds out both he and Ashley have been injected with the parasites of Las Plagas. Leon travels through a Spanish Village but is under attack by it's viscous residents, and soon encounters Ada Wong, one of his old friends from the Raccoon City Incident. President's daughter, Ashley Graham, from a mysterious cult known as the Los Illuminados led by Osmund Saddler.